Visualization of intermediate and transition-state structures in protein-tyrosine phosphatase catalysis.
نویسندگان
چکیده
منابع مشابه
Evidence for Protein - tyrosine - phosphatase Cysteine - Phosphate Intermediate
A recombinant protein-tyrosine-phosphatase has been expressed in Escherichia coli and purified to a single band by sodium dodecyl sulfate-polyacrylamide gel electrophoresis using affinity chromatography. When the phosphatase was allowed to react with 32Plabeled substrates and then rapidly denaturated, a 32Plabeled phosphoprotein could be visualized by sodium dodecyl sulfate-polyacrylamide gel ...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1996
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.93.6.2493